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Title	Evaluation of an Immunochromatographic Strip (Xenostrip –Tv) Test for Diagnosis of Vaginal Trichomoniasis Compared with Wet Mount and PCR Assay
Authors	*S Maraghi 1, A Khosravi 2, T Kardooni 1
Address	1Dept. of Parasitology and Mycology, Jondi-Shapour University of Medical Sciences, Ahwaz, Iran 2 Microbiology Dept. Tropical and Infectious Diseases Research Centre, Jondi-Shapour University of Medical Sciences, Ahwaz, Iran
Presenter	* Tel: +98-611-3738319       E-mail: maraghis@gmail.com
Background	The aim of this study was evaluation of immunochromatographic strip test (Xenostrip-Tv) for diagnosis of vaginal trichomoniasis compared with wet mount and PCR assay.
Methods	In this prospective study, vaginal swab samples were taken from 100 women with genital complaints demanding a speculum examination, referred to Imam Khomeini and Amir Kabir hospitals in Ahwaz, Khuzestan Province. Samples were first examined by wet mount and Xenostrip-Tv. PCR assay was performed in the next step using TVK3 and TVK7 primers initially. The positive samples were then confirmed by the second PCR assay using TVA5-1 and TVA6 primers.
Results	PCR with TVA5-1 and TVA6 primers was determined as gold standard. The wet mount as well as Xenostrip-Tv sensitivity and specificity were 73.3% and 100%, respectively in comparison with gold standard. The sensitivity and specificity of PCR with primers TVK3 and TVK7 were also determined as 100% and 96.6%, respectively. The infection rates were 14% for wet mount and Xenostrip-Tv, 21% for PCR with primers TVK3 plus TVK7 and 19% with the gold standard PCR using TVA5-1 and TVA6 primers.
Conclusion	Xenostrip- Tv could be used for diagnosis of vaginal trichomoniasis in regions with no laboratory diagnostic facilities.
Keywords	Trichomonas vaginalis, Xenostrip-Tv, Wet mount, PCR